Composite

Part:BBa_K3510007

Designed by: team work   Group: iGEM20_Tuebingen   (2020-10-21)


Anderson-Promoter-B12-Riboswitch-Tet-Inverter-System-mRFP-Terminator

Important: This construct does not include a RBS upstream of the mRFP sequence. When building on our design please make sure you add it in order to allow for translation

This composite part is a Vitamin B12 inducible expression system of a mRFP (BBa_E1010). The included part BBa_K1913008 contains a constitutive Promoter (BBa_J23100) and a riboswitch. Upon binding of Vitamin B12, the riboswitch prevents translation of the following gene. To express mRFP in the presence of Vitamin B12, a Tet inverter system (BBa_Q04400) is placed in between. When the encoded repressor is not translated anymore, the mRFP is expressed again. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). This part should have replicated the biobrick BBa_K1913011 by Team Wageningen 2016.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
    Illegal AgeI site found at 1929
    Illegal AgeI site found at 2041
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None