Part:BBa_K3507003
Antitoxin part of a toxin-antitoxin system of B. subtilis
YqcF is a small protein acting as an antitoxin in a type II toxin-antitoxin (TA) system described in Bacillus subtilis. This protein is capable of binding to its counterpart YqcG, thereby preventing its DNase activity in the cell. Most likely, this interaction occurs through binding to the LXG domain of YpcG (Holberger et al., 2012). However, it has been shown that it doesn’t exhibit antitoxic effects on other toxins that contain the same LXG domain (Holberger et al., 2012). For our iGEM project we intend on expressing this antitoxin under the control of a solanine dependent promoter with the purpose of rescuing cells that are located in the proximity of the potato root exudate. Solanine is a main and specific component of potato root exudates and, by putting the production of YpcF under the control of solanine dependent promoter, we can only allow its production in the potato environment. The cells will constitutively express the toxin part of the system (YqcG) but their survival will be dependent on the presence of solanine. By this, we hope to implement a kill switch system that allows the B. mycoides to survive only in the designated area.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 519
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Bibliography
1.Holberger LE, Garza-sánchez F, Lamoureux J, Low DA, Hayes CS. A novel family of toxin / antitoxin proteins in Bacillus species. FEBS Lett [Internet]. 2012;586(2):132–6. Available from: http://dx.doi.org/10.1016/j.febslet.2011.12.020
2.Brantl S, Müller P. Toxin-antitoxin systems in bacillus subtilis. Toxins (Basel). 2019;11(5).
Modifications in Sequence : iGEM21_IISER-Tirupati_India
BBa_K3889093 is a modified version specific to our project's assembly. Mutated BgIII site to make assembly compatible part.
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