Reporter

Part:BBa_K3500001

Designed by: Kennex Lam   Group: iGEM20_GA_State_SW_Jiaotong   (2020-10-26)


GUS Gene Codon Optimized for S. microadriaticum

GUS gene which encodes the enzyme glucuronidase has been added to DinoIII plasmid which is specific for Dinoflagellate as a reporter gene. GUS gene is a good reporter gene in experiments that are conducted on plants, the success of transformation can be observed via the visual color change for the first time by applying a histochemical technique, termed GUS staining, to the transformants. In addition, DinoIII is a plasmid peculiar to Dinoflagellate such as Symbiodinium, which contains the original promoter belongs to Dinoflagellate, which to a certain extent will benefit heterologous gene expresses.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 370
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 1099
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 16
    Illegal BsaI.rc site found at 1051
    Illegal BsaI.rc site found at 1147
    Illegal BsaI.rc site found at 1435
    Illegal SapI.rc site found at 1731


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