Composite

Part:BBa_K3498028

Designed by: Raúl Acosta Murillo   Group: iGEM20_FCB-UANL   (2020-09-25)


B. subtilis Sporulation Inhibitor

This piece has the function of decreasing the sporulation that the bacteria may have, since it is regulated by the concentration of the molecule Spo0A-A which activates the sporulation when it is in high concentration. In this way the bacteria can produce biofilm, however, if the concentration of this molecule increases, it will decrease to the point that it will not be able to sporulate. This piece has an adjustable promoter with a region of anchorage to the ribosome with a Spo0E protein, responsible for deforphorilizing Spo0A-P and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None