Composite

Part:BBa_K3498025

Designed by: Raúl Acosta Murillo   Group: iGEM20_FCB-UANL   (2020-09-25)


B. subtilis surfacin production enhancer

This piece allows increasing the production of surfactant generated by the B. subtilis by promoting this production and inhibiting the promoter inhibitor (RapC). This piece contains an adjustable promoter from xylose sugar, two coding regions and two ribosome anchorage sites and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1662
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1662
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1662
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1662
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2384
    Illegal SapI.rc site found at 2394


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Categories
Parameters
None