Coding
TdcE

Part:BBa_K348000

Designed by: Laurence Van Melderen   Group: iGEM10_ULB-Brussels   (2010-10-27)


TdCE - pyruvate formate lyase

2-ketobutyrate formate-lyase/pyruvate formate-lyase 4.

It catalyzes the reversible conversion of pyruvate and coenzyme-A into formate and acetyl-CoA; the formate can then be used for Hydrogen production. It is activated under anaerobic conditions, we wanted to constitutively express it in order to improve the formate production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1107
    Illegal AgeI site found at 511
    Illegal AgeI site found at 1861
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
None