Part:BBa_K3477007

Gene coding for his-tagged PEG-DH enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 te

This part constitutes the translational unit of polyethylene glycol dehydrogenase. Upstream of the coding region there is the biobrick prefix, the BBa_J23106 constitutive promoter (from the Anderson family) and the BBa_B0034 ribosome binding site (RBS). The gene sequence comes from Uniprot.org and is codon optimized for Escherichia coli. The sequence is followed by a 6x his-tag sequence. Downstream of the coding region there is the BBa_B0010 terminator and the biobrick suffix.

The enzyme coded for, PEG-DH, degrades polyethylene glycol into ethylene glycol. The his-tag can be used to purify the enzyme using a his-tag purification kit.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1784
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 56
  Illegal PstI site found at 659
  Illegal PstI site found at 795
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal NheI site found at 1419
  Illegal SpeI site found at 1785
  Illegal PstI site found at 659
  Illegal PstI site found at 795
  Illegal PstI site found at 1799
  Illegal NotI site found at 7
  Illegal NotI site found at 1792
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal BamHI site found at 896
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1785
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 56
  Illegal PstI site found at 659
  Illegal PstI site found at 795
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal XbaI site found at 56
  Illegal SpeI site found at 1785
  Illegal PstI site found at 659
  Illegal PstI site found at 795
  Illegal PstI site found at 1799
  Illegal AgeI site found at 1403
  
• 1000
  COMPATIBLE WITH RFC[1000]