Part:BBa_K3477003
Gene coding for his-tagged BphD enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 term
This part constitutes the translational unit of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD). Upstream of the coding region there is the biobrick prefix, the BBa_J23106 constitutive promoter (from the Anderson family) and the BBa_B0034 ribosome binding site (RBS). The gene sequence comes from Uniprot.org and is codon optimized for Escherichia coli. The sequence is followed by a 6x his-tag sequence. Downstream of the coding region there is the BBa_B0010 terminator and the biobrick suffix.
The enzyme coded for, BphD, originates from Pseudomonas sp. KKS102 and takes part in the first step of the Biphenyl degradation pathway. The Biphenyl degradation pathway degrades biphenyl molecules to benzoate and 2-oxopent-4-enolate. The included his-tag allows for purification of the expressed protein using a his-tag purification kit.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1010
Illegal EcoRI site found at 1
Illegal XbaI site found at 56 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 27
Illegal NheI site found at 50
Illegal SpeI site found at 1011
Illegal PstI site found at 1025
Illegal NotI site found at 7
Illegal NotI site found at 1018 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 1011
Illegal EcoRI site found at 1
Illegal XbaI site found at 56 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 56
Illegal SpeI site found at 1011
Illegal PstI site found at 1025
Illegal AgeI site found at 465 - 1000COMPATIBLE WITH RFC[1000]
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