![](https://parts.igem.org/images/partbypart/icon_t7.png)
T7
Part:BBa_K3476011
Designed by: Silvio Salvatore Bonni Group: iGEM20_MSP-Maastricht (2020-10-19)
bacterial shRNA_expression_cassette
Allows for the expression of hairpin RNA-sequences. The part can be used in T7-polymerase expressing, RNAseIII free bacterial strains. ShRNA coding DNA-sequences can be cloned in as a dsDNA-oligos using the EcoR1 and BamH1 restriction sites. Multiple cassettes coding different RNAs can be chained together using Gibson assembly. Expression is under IPTG-induction.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 56
Illegal NheI site found at 116
Illegal NotI site found at 31 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 56
Illegal BamHI site found at 62 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 56
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//direction/forward
//function/structures
//plasmid/expression/t7
transcriptional
//function/structures
//plasmid/expression/t7
transcriptional
None |