Part:BBa_K3463019

Thanks to the Standard Assembly technique, the two parts described BBa_K3463008 J23100 RBS RhlR B0015, and BBa_K3463011 PrhlR RBS eGFP were cloned in the same vector to obtain the plasmid pUCBB J23100-RhlR-B0015-PrhlR-eGFP. Indeed, by digesting J23100-RhlR-B0015 with EcoRI/SpeI, PrhlR-eGFP with XbaI/PstI and the backbone pUCBB with EcoRI/PstI, we built a new part able of both producing RhlR and sensing BHL. Therefore, 6 different growing cultures of the engineered E.coli Nissle were set up with a gradient of concentration of synthetic BHL : 100µM / 10µM / 1µM / 100nM / 10nM / without BHL. In addition, a negative control E.coli Nissle wild type was also used.