Composite

Part:BBa_K3463005

Designed by: Sara Salhi   Group: iGEM20_Grenoble_Alpes   (2020-10-22)


J23106 RBS eGFP

For our system to be as sensitive as possible to BHL, we needed to have the highest production of RhIR in the engineered E. coli strain. For that we tested three constitutive promoters with different strengths found in the iGEM Bank: J23100, J23102 and J23106. To identify the strongest one, we integrated the promoters into pUCBB-eGFP.

Then, we monitored the expression of the eGFP by measuring both the fluorescence and the absorbance of the bacterial cultures (transformed E. coli DH5 alpha). Thus we quantify the level of expression of eGFP as a function of time for the different constructs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None