Coding

Part:BBa_K3463001

Designed by: Elouen Le Garrec   Group: iGEM20_Grenoble_Alpes   (2020-10-22)


Pyocin S5

Bactericidal protein that uses the FptA ferripyochelin receptor to kill Pseudomonas aeruginosa. Has a C-terminal pore forming domain. The gene was cloned into a pUCBB plasmid (BBa_K3463000) and transformed into Escherichia coli for constitutive expression

SOurce:Genomic extraction by PCR on Pseudomonas aeruginosa PA01 strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None