Part:BBa_K346089

T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)

This part conbined MerT, MerP and MerC together under the regulation of T7 promoter. MerT,merP and merC can act together to transport Hg(II) into the cytosol to activate promoters which are dependent on intracellular Hg(II). In our bioabsorbant part, we designed this part to fulfill the function that more Hg(II) will be delivered into the cytosol so that the mercury responsive promoter(PmerT) will be activated to a great extent.It then will drive downstream gene's expression with a great expression level. In this way we have made sure the proteins that were used to bind Hg(II) are expressed in large quantity.

Figure 1: The function of merT,merP and merC in the transporting of Hg(II).

Figure 2: The cloned plasmid structure of merT merP and merC.

Reference

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1.Tamar B & Susan M(2002).FEMS Microbiology Reviews.27.355-384.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 501
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 86
  Illegal NgoMIV site found at 673
  Illegal NgoMIV site found at 1094
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 79