Generator

Part:BBa_K346084

Designed by: Weiye Wang   Group: iGEM10_Peking   (2010-10-19)

T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0015)

This part is used to generate T3 polymerase under T7 promoter. By constructing this part, we were able to test the level of different T3 promoter by inductive expressing T3 polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2637


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Categories
Parameters
None