Device

Part:BBa_K346058

Designed by: Heng Pan   Group: iGEM10_Peking   (2010-10-18)

T7promoter(BBa_I719005)+RBS(B0034)+phiR73 delta(BBa_I746352)+Terminator(B0015)+PO promoter(BBa_I7463

The device is designed for time delay and amplification. The inducement causes the expression of T7 polymerase which is extremely promoter specific. Then phiR73 delta downstream of T7 promoter is expressed, and this is the activator which can activate the gene downstream of PO promoter. The signal is amplified since the T7 polymerase - promoter pair is highly efficient. The signal is delayed because of the transcription and translation process of phiR73 delta. PO promoter's activation also takes time. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None