Generator

Part:BBa_K346038

Designed by: miao jing   Group: iGEM10_Peking   (2010-10-15)

PmerT promoter+T3 polymerase

PmerT promoter is activated by intracellular Hg(II).When merR dimer bind Hg(II) in the cytosol, it will bind to PmerT and activate it, so the gene downstream will be expressed. T3 RNA polymerase can regulate downstream T3 promoter, which activate target gene's expression.By constructing these two parts together, we are able to regulate and express gene under T3 promoter, and this expression is indirectly regulated by intracellular Hg(II).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2650


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