Generator
Part:BBa_K346038
Designed by: miao jing Group: iGEM10_Peking (2010-10-15)
PmerT promoter+T3 polymerase
PmerT promoter is activated by intracellular Hg(II).When merR dimer bind Hg(II) in the cytosol, it will bind to PmerT and activate it, so the gene downstream will be expressed. T3 RNA polymerase can regulate downstream T3 promoter, which activate target gene's expression.By constructing these two parts together, we are able to regulate and express gene under T3 promoter, and this expression is indirectly regulated by intracellular Hg(II).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2650
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Categories
Parameters
None |