Part:BBa_K346034
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)
This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide).It functions as the surface display part of MBP in our project.
Description
It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.
Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.
Figure 1: The structure of lpp-OmpA-MBP. This part can deliver the MBP to the surface and MBP will bind mercury more efficient and effective.
Figure 2: The expression of lpp-OmpA-MBP in the surface of our bacterial. We can see a clear band about 27KD in both SDS-PAGE and Western blotting.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 660
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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