Regulatory

Part:BBa_K3428003

Designed by: Andrew Sillato   Group: iGEM20_Stony_Brook   (2020-10-26)


UVB Light Responsive Promoter

The light responsive promoter is a gene promoter which drives expression of a downstream gene in the presence of a light-responsive activator. It consists of four tetO operator sequences (BBa_K105020) downstream of the core of the 35s CaMV promoter (BBa_K1825004). This promoter is designed to be employed along with a UVR8-TetR/COP1-VP16 optogenetic pair to induce transcription of a synthetic tasiRNA which knocks down expression of the WUSCHEL gene, a key gene responsible for stem cell maintenance in the shoot apical meristem of N. benthamiana.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 120


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Categories
Parameters
None