Part:BBa_K3428002
TetR-UVR8 fusion protein
TetR-UVR8 is a fusion protein consisting of UV RESISTANCE LOCUS 8 (UVR8) photoreceptor (BBa_K3428000) from Arabidopsis thaliana joined to a tetracycline repressor domain, abbreviated TetR by 3X Gly-4-Ser linker (BBa_K416001). Upon nuclear import, the TetR portion of the UVR8-VP16 fusion protein is constitutively bound to the tetO operator sequence [1]. Upon UV-B irradiation (~311 nm), the UVR8 homodimer undergoes a conformational change and dissociates [2]. Upon monomerization, the C-terminal tail of UVR8 adopts an extended conformation which facilitates binding to the WD40 domain of COP1 [3]. Upon the association of COP1-VP16 with UVR8-TetR, the downstream gene becomes activated. For the purposes of the 2020 Stony Brook iGEM team, this system will be used to facilitate the transcription of a synthetic ta-siRNA which knocks down expression of the WUSCHEL gene governing stem cell maintenance in the shoot apical meristem of N. benthamiana.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 736
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 436
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 70
Illegal SapI site found at 751
Illegal SapI.rc site found at 466
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