Composite
pyeaR GFP

Part:BBa_K3425131

Designed by: Emelie B. & Therese K.   Group: iGEM20_UofUppsala   (2020-10-21)


PyeaR GFP

This composite part has a codon optimized GFP (BBa_K3425132) under the control of the yeaR promoter (BBa_K3425130). In the presence of nitrates or nitrites, the E. coli NarX-NarL two-component system is activated, which in turn upregulates PyeaR. This triggers expression of the GFP, resulting in a visual color change.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/fluorescence
Parameters
biologyE. coli