Composite

Part:BBa_K3425059

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)


Juniper GFP template for amplification by Qβ replicase

BBa_K3425059 is a Juniper GFP template for amplification by Qβ replicase constructed from the constitutive promoter, BBa_J23108, the Qβ5' recognition sequence of Qβ replicase and RBS, BBa_K3425052, Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the Qβ3' recognition sequence of Qβ replicase with terminator, BBa_K3425053.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 567
    Illegal XbaI site found at 100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 567
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 567
    Illegal BglII site found at 103
    Illegal BamHI site found at 776
    Illegal XhoI site found at 107
    Illegal XhoI site found at 907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 567
    Illegal XbaI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 567
    Illegal XbaI site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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