Regulatory

Part:BBa_K3425035

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-16)


T7M5, weaker promoter of T7 RNA polymerase

BBa_K3425035 is a weaker version of the T7 promoter, the specific promoter of the T7 RNA polymerase. Sequence of this part was retrieved from the publication (1).

References
(1) Zong, Y., Zhang, H. M., Lyu, C., Ji, X., Hou, J., Guo, X., Ouyang, Q., and Lou, C. (2017) Insulated transcriptional elements enable precise design of genetic circuits. Nat. Commun. 8, 52

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote
//chassis/prokaryote/ecoli
//promoter
Parameters
controlorthogonal, promoter
directionforward
functiontranscription initiation