Part:BBa_K3423000

Anti-CRISPR (acr) promoter inhibitor

This anti-CRISPR-associated 1 (Aca1) protein is the inhibitor of Anti-CRISPR (acr) promoter, it was identified in 2019 (Stanley et al., 2019).

Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.

We characterised the efficiency of this part of inhibiting a downstream promoter, it successfully worked in our composite part BBa_K3423005.The inhibition of Aca1 is measured through green fluorescent protein:

Reference

Stanley, S. Y., Borges, A. L., Chen, K.-H., Swaney, D. L., Krogan, N. J., Bondy-Denomy, J., & Davidson, A. R. (2019). Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription. Cell, 178(6), 1452-1464.e1413. doi:10.1016/j.cell.2019.07.046

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]