Coding

Part:BBa_K342002

Designed by: Anne Chevrel   Group: iGEM10_INSA-Lyon   (2010-10-24)

Phasin-Phasin Intein (for silver standard fusion)

It's an association of two Phasin proteins with a self cleaving protein called Intein. The Phasin protein have the property to insert itself in the membrane of the PHB drop. The Intein protein cleaves itself when pH switches to 6.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1693
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1279
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None