Composite

Part:BBa_K3416201

Designed by: Eglė Vitkūnaitė   Group: iGEM20_Vilnius-Lithuania   (2020-10-22)


Kill-Switch inducable with AI-2

Introduction

FlavoFlow

Vilnius-Lithuania iGEM 2020 project FlavoFlowincludes three goals towards looking for Flavobacterium disease-related problems’ solutions. The project includes creating a rapid detection kit, based on HDA and LFA, developing an implement for treating a disease, and introducing the foundation of edible vaccines. This part was used for the second goal- treatment - of the project FlavoFlow.

FlavoFlow

Biology

Quorum sensing (QS) is a communication system, which controls gene expression in response to population density, between bacteria. There are two QS systems: the first one, based on AI-1 or acyl-homoserine lactone (AHL), and the second – autoinducer 2 [1].

AI-2 - interconverting molecules, which are derived from the same precursor and called the „universal“ bacterial signal [1], [2], [3]. AI-2 controls the expression of LuxS regulated transporter, which is responsible for incorporation, phosphorylating, and processing of the AI-2 signal. The lsr transporter has genes, which expression is regulated by AI-2. LsrR is a repressor of the lsr operon. The AI-2, phosphorylated by lsrK, leads to derepression of lsr operon [4].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 642
    Illegal NheI site found at 665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ 1.0 1.1 Stephens, K. & Bentley, W. E. Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia. Trends in Microbiology 28, 633–643 (2020).
  2. ↑ Sun, J., Daniel, R., Wagner-Döbler, I. & Zeng, A.-P. Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways. BMC Evol Biol 4, 36 (2004).
  3. ↑ Xavier, K. B. & Bassler, B. L. Interference with AI-2-mediated bacterial cell-cell communication. Nature 437, 750–753 (2005).
  4. ↑ Pei, D. & Zhu, J. Mechanism of action of S-ribosylhomocysteinase (LuxS). Current Opinion in Chemical Biology 8, 492–497 (2004).
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