Translational_Unit

Part:BBa_K341005

Designed by: Danyang He   Group: iGEM10_Tsinghua   (2010-10-20)


recombination sequence + Kan+I-Sce I cutting site

Recombination sequence 1 and 2, which is homologous to part of modified E.coli genome, the CDS of gene Kan and I-Sec I cutting site are all included in the MCS of PSB1C13 plasmid. The biorick is designed to verify the "landing pad method" we use in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 238
    Illegal NgoMIV site found at 689
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 538
    Illegal SapI.rc site found at 748


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