Translational_Unit

Part:BBa_K341004

Designed by: Danyang He   Group: iGEM10_Tsinghua   (2010-10-20)


recombination sequence +mCherry+I-Sce I cutting site

Recombination sequence 1 and 2, which is homologous to part of modified E.coli genome, the CDS of gene mCherry and I-Sec I cutting site are all included in the MCS of PSB1C13 plasmid. The biorick is designed to verify the "landing pad method" we use in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 390
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 726
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 390
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 390
  • 1000
    COMPATIBLE WITH RFC[1000]


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