Translational_Unit
Part:BBa_K341004
Designed by: Danyang He Group: iGEM10_Tsinghua (2010-10-20)
recombination sequence +mCherry+I-Sce I cutting site
Recombination sequence 1 and 2, which is homologous to part of modified E.coli genome, the CDS of gene mCherry and I-Sec I cutting site are all included in the MCS of PSB1C13 plasmid. The biorick is designed to verify the "landing pad method" we use in our project.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 390
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 390
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 726
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 390
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 390
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |