Part:BBa_K3408005

Optimized DpnI

It is an enzyme which could cut all methylated DNA so that engineered bacteria may die in a certain situation. It could be used to ensure bio-safety.

Bacillus subtilis has been increasingly applied in genetic engineering due to its powerful secretion capacity.

As a restriction enzyme which is capable of cutting all methylated DNA, optimized DpnI via codon optimization can be applied in Bacillus subtilis more efficiently.

                                 Fig.1. Codon quality distribution                                              Fig.2. Optimized codon quality distribution

The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).

                                 Fig.3. Codon quality plot                                                         Fig.4. Optimized codon quality plot

The plots show the quality of the used codon at the indicated codon position.

                                 Fig.5. GC content                                                                     Fig.6. Optimized GC content

The plots show the GC content in a 40 bp window centered at the indicated nucleotide position.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1322
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 210
  
• 1000
  COMPATIBLE WITH RFC[1000]