Regulatory

Part:BBa_K3407013

Designed by: Javier Navarro Delgado   Group: iGEM20_TUDelft   (2020-10-23)


T7 truncated promoter

T7 promoter lacking the last two GG. This is necessary to transcribe shRNA (BBa_K3407006) and shRNA* (BBa_K3407007) in vitro with RiboMAX express RNAi kit (Promega), as the template of the shRNA already has to be desinged by starting with GG. When coupled together, the transcript will be exactly what is designed as the shRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None