Regulatory

Part:BBa_K3402046

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-22)


Pcyp52m1

Pcyp52m1 is the promoter of Cytochrome P450 monooxygenase from S. bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 638
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 257
    Illegal AgeI site found at 1097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 286


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Parameters
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