Coding

Part:BBa_K3402011

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-01)


UGTB

UGTB, the gene of glucosyltransferase in Starmerella bombicola, is responsible for the second glycosylation step, verified by constructing UGTB gene deletion strain and measuring extracellular enzyme solution.

Usage and Biology

There are two independent UDP-glucosyltransferases existing during the synthesis of sophorolipids, UGTA and UGTB. UGTA is mainly responsible for the first step of glycosylation, and the knockout of this gene has no effect on the second step of glycosylation, but no sophorolipid is detected in the product. When UGTB was knocked out, sophorolipid was not detected in the product, but glucolipid could be detected. So UGTB is the key gene in the synthesis of sophorolipid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 415
    Illegal BsaI.rc site found at 370


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Categories
Parameters
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