Regulatory

Part:BBa_K3402002

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-01)


Ppgi

Ppgi is the promoter of glucose-6-phosphate isomerase from Starmerella bombicola.

Usage and Biology

We used green fluorescence protein yeGFP (BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Ppgi by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 259
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 259
    Illegal BglII site found at 837
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 259
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None