Part:BBa_K339015

Usage and Biology

Red fluorescent protein (RFP) is already present in the registry however this RFP is a variant that can localize in the cytoplasm as well as the outer membrane. Regular RFP requires specific chaperone proteins that are only present in the cytoplasm. The limitation of this as a reporter gene is that it would not produce a visual output if the protein of interest is sent to the periplasm. We introduced this sequence to the registry because this RFP is capable of folding without the presence of those chaperone proteins. We use this special RFP (sRFP) in our transcription/translation circuit by fusing the sRFP gene to the GOI. In this manner this circuit can now detect translation in both the cytoplasm and periplasm.

Reference:

Lewenza, S., Vidal-Ingigliardi, D., & Pugsley, A. P. (2006). Direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family Enterobacteriaceae. Journal of bacteriology, 188(10), 3516-24.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]