Composite

Part:BBa_K339009

Designed by: Christopher Tang   Group: iGEM10_Calgary   (2010-10-25)

CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31

The CpxR promoter driving expression of red fluorescent protein (RFP). Downstream of this is an arabinose inducible promoter driving the expression of mutant maltose binding protein (malE31) with a storng ribosomal binding site.


Usage and Biology

malE31 is a mutant form of the maltose binding protein, a native protein to Escherichia Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2603
    Illegal BamHI site found at 2076
    Illegal BamHI site found at 2339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2301
    Illegal SapI site found at 1893


[edit]
Categories
Parameters
None