Coding
Part:BBa_K3382002
Designed by: Leung Chung Ki Group: iGEM20_HK_CPU-WFN-WYY (2020-10-14)
Thermobifida fusca KW3 cutinase
TfCut2 is a cutinase found in Thermobifida fusca KW3. It can degrade PET and form terephthalic acid and ethylene glycol as its major product. TfCut2 degrades PET film with a weight loss of more than 50% after 96 hours of incubation at 70 degrees celsius. Ca2+ and Mg2+ ions work as a mediator of TfCut2. TfCut2 can also fully degrade consumer PET materials after being mechanically treated to reduce the diameter of PET particles to about 100 nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |