Coding

Part:BBa_K3382002

Designed by: Leung Chung Ki   Group: iGEM20_HK_CPU-WFN-WYY   (2020-10-14)


Thermobifida fusca KW3 cutinase

TfCut2 is a cutinase found in Thermobifida fusca KW3. It can degrade PET and form terephthalic acid and ethylene glycol as its major product. TfCut2 degrades PET film with a weight loss of more than 50% after 96 hours of incubation at 70 degrees celsius. Ca2+ and Mg2+ ions work as a mediator of TfCut2. TfCut2 can also fully degrade consumer PET materials after being mechanically treated to reduce the diameter of PET particles to about 100 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None