Part:BBa_K3379002

Laccase Trametes versicolor with 3xlys tag and 6xhis tag

This part is based on the part BBa_K2835003 which was contributed by the iGEM Stockholm team in the year 2018. It´s a fungal laccase which is known for its ability to degrade lignin. It has a broad substrate range allowing it to degrade other phenolic substrates like pharmaceutics or antibiotics which accumulate in wastewater. This laccase was examined regarding its immobilization in a Mesocellular silica Foam (MCF) which could possibly improve lifetime and long-time activity of the resulting MCF-Laccase filter unit in comparison to the free enzyme. This could reduce costs and allow a feasible scaled up application of an immobilized enzyme wastewater treatment approach.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1107
  Illegal BglII site found at 1240
  Illegal BamHI site found at 1249
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]