Protein_Domain
LSc

Part:BBa_K3379001

Designed by: Erik M�ller, Nikolai Herdrich   Group: iGEM20_Stuttgart   (2020-10-18)


Laccase of Streptomyces cyaneus with 6xHis Tag and 6xLys Tag

Streptomyces cyaneus strain CECT 3335 laccase (sclA) gene. Coding region for a laccase of S cyaneus strain. These enzymes are known for its ability to degrade lignin. They often got a broader substrate spectrum which allows degradation of other phenolic compounds. Hence laccases are capable of degrading antibiotics, drugs and other substances which tend to remain undegraded by common wastewater treatment techniques. This laccase showed good results regarding activity (in ABTS-Assays) and stability and showed ability to degrade Diclofenac. This laccase could be a good addition to enzymatic wastewater treatment since there are often applicated fungal laccases which are often more expensive regarding costs and time of expression. This part includes a 6x his tag c-terminal as well as a 6x lys tag n-terminal to the protein coding region. The his tag should allow purification while the lys tag could possibly improve the immobilization process in Mesocellular silica Foam (MCF) through providing additional amino-groups.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1912
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 169
    Illegal SapI site found at 652
    Illegal SapI site found at 1027


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Categories
Parameters
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