Coding

Part:BBa_K3373005

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-16)


UVR8 A. thaliana codon optimized for C. reinhardtii

Coding sequence of the Ultraviolet-B receptor encoded by the gene UVR8 from Arabidopsis thaliana and codon optimized for Chlamydomonas reinhardtii. Upon UV-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer which leads to the interaction with the photomorphogenic repressor COP1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 850
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 28
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 28
    Illegal NgoMIV site found at 75
    Illegal AgeI site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None