Coding

Part:BBa_K3373003

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-13)


atzC P. sp. ADP codon optimized for C. reinhardtii

Coding sequence of the N-isopropylammelide isopropyl amidohydrolase enzyme from Pseudomonas sp. ADP codon optimized for Chlamydomonas reinhardtii. Catalyzes the hydrolysis reaction of N-isopropylammelide (dihydroxy-isopropyl-atrazine) to cyanuric acid and isopropylamine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 885
    Illegal XhoI site found at 993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None