Regulatory

Part:BBa_K337039

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-22)

TetR_mut(XhoI/XbaI)

Mutated TetR where XhoI/XbaI sites were introduced right after the stop codon enabling easy introduction of synthetic microRNA binding sites into the 3'UTR of the TetR (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 652
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None