Composite
Part:BBa_K337026
Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R Group: iGEM10_Heidelberg (2010-10-22)
BGH(rc)/shRNA6(rc)/sv40(rc)/CMV(fw)
SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter right behind SV40 drives the GOI in the opposite direction (fw). (BB-2, RFC12)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |