Other

Part:BBa_K337019

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-21)

FRT site with spacing

FRT sites can be used to stably integrate DNA parts into a host cell genome via the Flip-in System (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 155
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 155
    Illegal NgoMIV site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None