Composite

Part:BBa_K336061

Designed by: Ryuto Nitta   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)


promoter+RBS+ompA signal+lacZ+double termnator+promoter+RBS+SBFP2+double termnator

This device is mede up with BBa_K208017, BBa_I732005, BBa_B0012, BBa_B0011, BBa_R0010, BBa_B0030, BBa_K156010, BBa_B0012 and BBa_B0011. This device expresses CyaA (Adenylate cyclase) out of cytoplasm and mRFP1 in cytoplasm. This device have function of inhibiting transcription of the lac operon and show own expression level by coloring. The inhibiting system is catabolic repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3726


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Categories
Parameters
None