Composite

Part:BBa_K336060

Designed by: Ryuto Nitta   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)


promoter+RBS+ompA signal+cyaA+double termnator+promoter+RBS+mRFP1+double termnator

This device is mede up with BBa_K208017, BBa_K336040, BBa_B0010, BBa_B0012 and BBa_I13521. This device expresses CyaA (Adenylate cyclase) out of cytoplasm and mRFP1 in cytoplasm. This device have function of self activation and show expression level by col0ring. Because CyaA catalyzes the conversion of ATP to 3',5'-cyclic AMP (cAMP) and pyrophosphate and mRFP1. cAMP forms a complex with cAMP receptor protein (CRP) and thereby is activated to bind to DNA. CRP-cAMP increases expression of a large number of genes, including some encoding enzymes that can supply energy independent of glucose. The protein assumes its active shape and binds to a specific site upstream of the lac promoter, making it easier for RNA polymerase to bind to the adjacent promoter to start transcription of the lac operon, increasing the rate of lac operon transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None