Device

Part:BBa_K336050

Designed by: Ryuto Nitta   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)

promoter+RBS+cyaA+double termnator+promoter+RBS+crp+double termnator

This is the device that express CyaA and CRP and export CyaA out of the cytoplasm. Its has function of self activation and activate transcription of the lac operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 291
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 553
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 291
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1187
    Illegal SapI.rc site found at 677
    Illegal SapI.rc site found at 917


[edit]
Categories
Parameters
None