A T7 promoter from pet28a plasmid.
It is a strong promoter from T7 bacteriophage that can specifically react to T7RNA polymerase. It is a sequence that initiates the transcription of T7 bacteriophage gene.
Characterization by NWU-CHINA-A 2021
We used this part in our composite part BBa_K3722007, BBa_K3722008 and BBa_K3722009 to express tryptophanase (TnaA, BBa_K3722000), monooxygenase (MaFMO, BBa_K3722002) and tryptophan (Trp) halogenase (SttH, BBa_K3722003). All of them were constructed into pET-28a and transformed to E.coli (DE3) for expression.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
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- 1000COMPATIBLE WITH RFC