Reporter

Part:BBa_K334002

Designed by: Sean Kearney   Group: iGEM10_Purdue   (2010-10-25)


ODD with Luciferase

Part of the degradation domain taken from the HIF-1a protein in humans. Under normal oxygen conditions, prolyl hydroxylases target this domain for hydroxylation. The hydroxylated subunit makes the resultant protein a target for E3 ubiquin ligase when bound by the VHL gene product, leading to its proteasomal degradation. When fused to the luciferase, and following a strong, mammalian constitutive promoter, it should indicate low levels of oxygen in cell populations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 982


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Categories
Parameters
None