Protein_Domain

Part:BBa_K334000

Designed by: Sean Kearney   Group: iGEM10_Purdue   (2010-07-21)


Oxygen Dependent Degradation Domain

Part of the degradation domain taken from the HIF-1a protein in humans. Under normal oxygen conditions, prolyl hydroxylases target this domain for hydroxylation. The hydroxylated subunit makes the resultant protein a target for E3 ubiquin ligase when bound by the VHL gene product, leading to its proteasomal degradation. This can be used, when fused to a reporter protein, to indicate the activity of endogenous HIF-1a in real-time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None