Composite

Part:BBa_K3329068

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-spider fibroin*2-sfGFP for E. coli

SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The super-folder GFP protein is connected with spider fibroin. The green color indicates whether spider fibroin is bound to cellulose by seeing whether that filter paper turned green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2041
    Illegal NotI site found at 635
    Illegal NotI site found at 1700
    Illegal NotI site found at 1772
    Illegal NotI site found at 1790
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2453
    Illegal XhoI site found at 1979
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1936
    Illegal AgeI site found at 2932
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None