Composite

Part:BBa_K3329064

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyCatcher003*3-CBM*2-mRFP for E. coli

SpyCatcher003 is a sub part in the Spytag-catcher003 system. It should be used with SpyTag003. The cellulose binding module help connect cellulose and Spycatcher003. The monomeric red fluorescent protein is connected with CBM. The red color indicates whether the CBM is bound to cellulose by seeing whether that filter paper turned red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
    Illegal BglII site found at 744
    Illegal XhoI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1105
    Illegal AgeI site found at 2047
    Illegal AgeI site found at 2314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 390


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Categories
Parameters
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